GlykoPrep Technology
- N-Glycan Sample Preparation Kits
Product Details
- The GlykoPrep platform supports traditional glycan labels such as 2-AB and APTS, as well as the first commercially available instant label for glycans, InstantAB, and the innovative InstantPC.
- GlykoPrep is modular and can be integrated into any workflow. Kit components are also available individually as Digestion Module and dye-specific Labeling & Cleanup Modules, with optional AssayMAP PA50 module for purification of Fc-containing antibodies.
- GlykoPrep is built on AssayMAP technology - microchromatography in a 96-well format. In the manual protocol, GlykoPrep is performed using centrifugation to move liquid through the cartridges (spin format). The workflow is automatable on the Agilent AssayMAP Bravo, see GlykoPrep-plus kit webpages for more detail.
- Application Notes
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Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
- Application Notes
- English
- 11 Jul 2019
- 2.09 MB
A Comprehensive Approach for Monoclonal Antibody N-linked Glycan Analysis from Sample Preparation to Data Analysis
This application note demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent biopharmaceutical solution.
- Application Notes
- English
- 23 Apr 2019
- 3.70 MB
Profiling Glycosylation of Monoclonal Antibodies at Three Levels Using the Agilent 6545XT AdvanceBio LC/Q-TOF
This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.
- Application Notes
- English
- 23 Apr 2019
- 1.20 MB
Analysis of Monoclonal Antibody N-glycans by Fluorescence Detection and Mass Selective Detection Using LC/MSD XT
This app note demonstrates the performance of an Agilent LC/MSD to solve ambiguous peak assignments for the analysis of N-glycosylation of therapeutic proteins
- Application Notes
- English
- 14 Jan 2020
- 775.86 KB
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GlykoPrep Technology Frequently Asked Questions (FAQ)
Frequently asked questions for GlykoPrep Technology
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- View all AdvanceBio Product Citations Learn More
The Importance of Glycan Analysis and Characterization for Biopharma
Professor Michael Butler, head of the National Institute for Bioprocessing Research and Training (NIBRT) Cell Technology Group (CTG), explains that the characterisation and analysis of glycans is important for the biopharma sector as it can help in the development of therapeutic agents, such as monoclonal antibodies and other recombinant proteins. These are used in therapies in a range of serious conditions including cancer, inflammatory diseases, and cardiovascular diseases. The mechanism of action depends upon their strong affinity to molecular targets associated with disease.
- Drug Discovery World (DDW)
- 10 Oct 2022