Charge variants of monoclonal antibodies arise through different levels of glycosylation, deamidation, oxidation of amino acids, and lysine truncation of heavy chains. Analysis of these charge variants as part of an in-depth characterization is crucial to ensure safety and efficacy.
Identifying and quantifying charge variants in biotherapeutics require a finely tuned workflow that produces reliable, reproducible results.
The following resources and solutions will help you perform a complete, confident charge variant analysis.
This application note demonstrates the comparison between two rituximab biosimilars from different manufacturers to the innovator for their aggregation and charge variant profiles by following two analytical workflows using Agilent 1260 Infinity II bio-inert LC and Agilent AdvancedBio columns.
This application note demonstrates the evaluation different salt gradients on the Agilent 1290 Infinity II Bio LC System and analyzed for resolution as well as reproducibility.
In this application compendium, the Agilent solution suitable for the measurement of each CQA is discussed in the context of characterizing NISTmAb. This compendium also aims to provide guidance for the appropriate liquid chromatography columns for the different types of detection that may be required, and to provide a valuable reference for future consideration.
An ultra high performance biocompatible LC system to protect your biomolecules and offering flexibility
From routine analysis to cutting-edge research, the InfinityLab LC Series offers the broadest portfolio of HPLC and UHPLC solutions for any application and budget.
The LC/MS system that provides accurate mass measurement with high mass resolution for confident compound identification beyond the conventional HPLC detection.
A complete suite of ion exchange columns for charge variant analysis, including weak anion exchange, weak cation exchange, strong anion exchange, and strong cation exchange.
DE79534821
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